Journal: EMBO Reports

Article Title: SIRT4 regulates antiviral and autoimmune responses by promoting cGAS-mediated signaling pathways

doi: 10.1038/s44319-026-00708-5

Figure Lengend Snippet: ( A ) Sex and age-matched wild-type (WT) ( n = 8) and Sirt4 -deficient (KO) ( n = 6) mice (8-week-old) were intravenously infected with HSV-1 (2 × 10 7 PFU). The survival of these mice was monitored for 15 days. Each drop in the survival curve represents a death event. Log-rank (Mantel–Cox) test was used for statistical analysis. ( B ) Sex and age-matched wild-type (WT) ( n = 6) and Sirt4 -deficient (KO) ( n = 5) mice (8-week-old) were intravenously infected with HSV-1 (1 × 10 7 PFU). The body weight loss was monitored for 12 days. Two-way ANOVA was used for statistical analysis. ( C ) Levels of IFN-β, and IL-6 in serum from wild-type (WT) and Sirt4 -deficient (KO) mice ( n = 10, 8-week-old) were measured by ELISA 6 h after intravenous infection with HSV-1 (1 × 10 7 PFU). Results are mean ± SD. Each data point represents one mouse. Unpaired t test was used for statistical analysis. ( D ) Wild-type (WT) and Sirt4 -deficient (KO) mice ( n = 10, 8-week-old) were intranasally infected with HSV-1 (1 × 10 7 PFU) for 24 h. Bronchoalveolar lavage fluid (BALF) was collected and analyzed by ELISA. Results are mean ± SD. Each data point represents one mouse. Unpaired t test was used for statistical analysis. ( E , F ) Wild-type (WT) and Sirt4 -deficient (KO) mice ( n = 10) were intravenously infected with HSV-1 (1 × 10 7 PFU) for 24 h. Lungs, livers, and spleens were then harvested and analyzed by ELISA ( E ) or real-time PCR ( F ). Results are mean ± SD. Each data point represents one mouse. Unpaired t test was used for statistical analysis. ( G ) Wild-type (WT) and Sirt4 -deficient (KO) mice ( n = 10) were intravenously infected with HSV-1 (1 × 10 7 PFU) for 4 days. Brains were then harvested and analyzed by real-time PCR assays. Results are mean ± SD. Each data point represents one mouse. Unpaired t test was used for statistical analysis. ( H ) Sex and age-matched wild-type (WT) and Sirt4 -deficient (KO) mice were intravenously infected with HSV-1 (1 × 10 7 PFU) for 24 h. Lung sections were analyzed by H&E staining. Scale bars, 1000 μm (left), 50 μm (right). One representative image for each group is shown. ( I ) Wild-type (WT) and Sirt4 -deficient (KO) mice ( n = 9, 8-week-old) were intranasally infected with VSV (5 × 10 7 PFU) for 24 h. BALFs were collected and analyzed by ELISA. Results are mean ± SD. Each data point represents one mouse. Unpaired t test was used for statistical analysis. .

Article Snippet: THP1 cells were infected with viruses or transfected with synthetic nucleic acids for 24 h. Supernatants were collected to measure IFN-β (KE00187, Proteintech) and TNF-α (88-7346-88, Thermo Fisher Scientific).

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Staining

Journal: Cell Research

Article Title: Transient mechanical activation of the Piezo1 channel facilitates ex vivo expansion of hematopoietic stem cells

doi: 10.1038/s41422-025-01209-1

Figure Lengend Snippet: a Schematic (left) and PCA analysis (right) of RNA-seq, n = 3. b KEGG analysis of upregulated genes in HSCs after a 3-day PS500 co-culture. c GSEA enrichment plots showing downregulated (top) and upregulated (bottom) genes in Jak1 KO HSCs, enriched in PS500-cultured HSCs. NES, normalized enrichment score; FDR, false discovery rate. d Expression heatmap of upregulated cytokines in PS500-cultured HSCs and their downstream activated Stats. e Heatmap showing relative expression of cytokine genes enriched in PS500-co-cultured WT HSCs quantified by qPCR, n = 3. f Heatmap showing the relative secretion of cytokines enriched in PS500-co-cultured supernatant as determined by a cytokine array, n = 3. g Ifn-γ and Il-6 concentrations in PS500-co-cultured supernatant, n = 3. h MFI of p-Stat1 (S727) and p-Stat3 (Y705) in WT HSCs after PS500 co-culture, n = 5. i Immunofluorescence visualizations of p-Stat3 (Y705) staining in WT HSCs co-cultured with PS500. Red, p-Stat3 (Y705); blue, DAPI. Scale bar, 2 μm. j Fold expansion of WT HSCs treated with 100 nM Ang, PS500, or both for 3 days, n = 3. k Schematic of NFAT reporter (left) and mCherry images in WT HSCs at 24 h post-PS500 co-culture (right). Scale bar, 10 μm. l MFI of mCherry in WT HSCs after PS500 co-culture, n = 3. m Schematic of PS500 short-term co-culture (left) and MFI of p-Stat3 (Y705) in HSCs at 72 h with PS500 co-culture for 12 h, 24 h, and 72 h (right), n = 5. n Fold expansion of HSCs at 72 h with PS500 co-culture for 12 h, 24 h, and 72 h, n = 5. o The PS500–HSC interaction generated transient Piezo1 activation via mechanical force, leading to Ca 2+ influx and NFAT-mediated cytokine expression. Il-6 signaling activates Stat3 and induces expression of HSC expansion-related genes. Data are shown as the mean ± SD. The statistical tests of RNA-seq data were one-sided, and adjustments were made for multiple comparisons. Statistical significance for other comparisons was assessed with a two-tailed, unpaired Student’s t -test. Exact P values are provided.

Article Snippet: The supernatants of cells from different treatments were centrifuged at 13,000× g for 10 min to remove cellular debris before ELISA measurements using kits for mouse IFN-γ (ThermoFisher Scientific), mouse IL-6 (BMS603-2, ThermoFisher Scientific), and human IL-6 (EK0410, Boster Bio).

Techniques: RNA Sequencing, Co-Culture Assay, Cell Culture, Expressing, Immunofluorescence, Staining, Generated, Activation Assay, Two Tailed Test